HaloTag8: an engineered protein tag improving fluorophore performance and enabling fluorescence lifetime multiplexing.

  • Date: May 6, 2021
  • Time: 03:00 PM (Local Time Germany)
  • Speaker: Michelle Frei
  • present position: Max-Planck-Institute for Medical Research in Heidelberg
  • Location: web-talk
  • Room: Zoom-Meeting
  • Host: Max Planck BioImaging Core Unit Network - Spotlight Talks
HaloTag8: an engineered protein tag improving fluorophore performance and enabling fluorescence lifetime multiplexing.
Meeting ID: 894 9575 9133 Passcode: 579505

Michelle Frei will talk about the following preprint:

HaloTag8: an engineered protein tag to improve fluorophore performance

Abstract

HaloTag8 is an engineered variant of HaloTag7 with up to 40% higher brightness and increased fluorescence lifetime when labeled with fluorogenic rhodamines. Moreover, combining HaloTag8 with HaloTag7 and other fluorescent probes enabled live-cell multiplexing using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. The increased brightness of HaloTag8 and its use in fluorescence lifetime multiplexing makes it a powerful tool for live-cell imaging.

Preprint

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