Regional specialization and fate specification of bone stromal cells in skeletal development

  • Date: Oct 21, 2021
  • Time: 15:00 - 16:15
  • Location: web-talk
  • Room: Zoom-Meeting
  • Host: Max Planck BioImaging Core Unit Network - Spotlight Talks
Regional specialization and fate specification of bone stromal cells in skeletal development
https://gwdg.zoom.us/j/89333942225?pwd=Rnl4QWp3V3JVYkduNmxZbmYxSDNJdz09 Meeting ID: 893 3394 2225 Passcode: 336008

Dr. Kishor Kumar Sivaraj will talk about the following paper:

Regional specialization and fate specification of bone stromal cells in skeletal development

Abstract

Bone stroma contributes to the regulation of osteogenesis and hematopoiesis but also to fracture healing and disease processes. Mesenchymal stromal cells from bone (BMSCs) represent a heterogenous mixture of different subpopulations with distinct molecular and functional properties. The lineage relationship between BMSC subsets and their regulation by intrinsic and extrinsic factors are not well understood. Here, we show with mouse genetics, ex vivo cell differentiation assays, and transcriptional profiling that BMSCs from metaphysis (mpMSCs) and diaphysis (dpMSCs) are fundamentally distinct. Fate-tracking experiments and single-cell RNA sequencing indicate that bone-forming osteoblast lineage cells and dpMSCs, including leptin receptor-positive (LepR+) reticular cells in bone marrow, emerge from mpMSCs in the postnatal metaphysis. Finally, we show that BMSC fate is controlled by platelet-derived growth factor receptor β (PDGFRβ) signaling and the transcription factor Jun-B. The sum of our findings improves our understanding of BMSC development, lineage relationships, and differentiation.

paper "Regional specialization and fate specification of bone stromal cells in skeletal development "

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