Enhancing the biocompatibility of rhodamine fluorescent probes by positional isomerism: developer's and user's perspective
- Date: Mar 31, 2022
- Time: 03:00 PM - 04:15 PM (Local Time Germany)
- Speakers: Ruta Gerasimaite and Jonas Bucevicius
- present positions at Max Planck Institute for Multidisciplinary Sciences
- Location: web-talk
- Room: Zoom-Meeting
- Host: Max Planck BioImaging Core Unit Network - Spotlight Talks
Ruta Gerasimaite and Jonas Bucevicius will talk about the following paper:
Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect
Abstract
Fluorescence microscopy is an essential tool for understanding dynamic
processes in living cells and organisms. However, many fluorescent
probes for labelling cellular structures suffer from unspecific
interactions and low cell permeability. Herein, we demonstrate that the
neighbouring group effect which results from positioning an amide group
next to a carboxyl group in the benzene ring of rhodamines dramatically
increases cell permeability of the rhodamine-based probes through
stabilizing a fluorophore in a hydrophobic spirolactone state. Based on
this principle, we create probes targeting tubulin, actin and DNA. Their
superb staining intensity, tuned toxicity and specificity allows
long-term 3D confocal and STED nanoscopy with sub-30 nm resolution. Due
to their unrestricted cell permeability and efficient accumulation on
the target, the new probes produce high contrast images at low nanomolar
concentrations. Superior performance is exemplified by resolving the
real microtubule diameter of 23 nm and selective staining of the
centrosome inside living cells for the first time.
Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect