Dr. Choongman Lee will talk about:

Targeting and Manipulating Endogenous Transcriptional Condensates

Abstract

Liquid-like biomolecular condensates are membraneless compartments composed of selectively concentrated biomolecules with liquid-like properties. Previously, we discovered that transcription is associated with the liquid-like condensation. However, tools to study transcriptional condensates in living cells are limited due to the size of the condensates (tens to hundreds of nanometers) and the transient dynamics of the condensates in living cells. Therefore, in this study, we develop an optogenetic tool to target and manipulate endogenous transcriptional condensates. We find that intrinsically disordered regions of transcription factors can be used to direct cargo to the condensates. Combined with an improved light-induced dimer (iLID) and its binding partner (sspB) allow us to generate a quick response with high insertion efficiency. We adopt a proximity-based modification (BioID) to biotinylate proteins inside of the condensates upon blue light exposure. This helps to determine the constituent of the condensates using mass spectrometry. Our approach opens the road to the proteome-wide investigation of the transcriptional condensates.

Zoom Meeting
Meeting ID: 669 0415 4170
Passcode: 659243