This is an online tutorial for the spectral separation of multiple fluorescences in confocal microscopy using the online-fingerprinting method at a Zeiss LSM780/880/980 confocal microscope. It will be shown how to prepare the spectra, how to setup the acquisition, as well as an example for a good sample and for a problematic one. After an explanation of a few pitfalls and possible artifacts we will end with a discussion round. Please follow the link in the details to register at the Max Planck Academy.
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